Which pipettes are recommended to work with the AmpliGrid?
We recommend Rainin AutoRepE or Eppendorf Multipette® stream. Only electronic pipettes ensure consistent pipetting of the PCR and sealing solutions without forming satellite droplets. If only manual pipettes are available, be careful not use the second pressure point of the pipette. Otherwise, you will inject small air bubbles into the reaction mix which can cause the mix to burst during cycling. Also avoid to deposit excessive amounts of buffer when depositing cells as the buffer crystals can lead to bubble formation.
