Which paint is best used to distinguish between human and mouse cells?
We recommend our Star*FISH Human Chromosome-specific 22 paint. This will pick out chromosome 22, which mice do not have. Alternatively, human and mouse centromerics should not cross-react, due to the divergence between species of the repetitive sequences. Otherwise, mouse and human Y probes do not cross react (you have to increase the hybridisation time from overnight to about 3 days to get cross-reactivity). However, if you’re not always using male cells, then this option is not suitable.