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Which packaging cell line should I use to make lentivirus?

April 26, 2017CELL lentivirus line packaging

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Which packaging cell line should I use to make lentivirus?

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Most cell lines are NOT ideally suited to produce lentivirus with the MISSION shRNA library. We suggest using an HEK-293T cell line for generating virus particles. In addition, you’ll need to use packaging vectors derived from HIV. We recommend using our Packaging Mix (Product No. SHP001) for optimal high titer virus production.