Which methods for manufacturing dNTPs exist?
dNTPs are normally manufactured from deoxynucleosides or deoxynucleoside monophosphates (dNMPs) by either chemical phosphorylation or enzymatic synthesis. Chemical synthesis involves the addition of phosphate groups or inorganic pyrophosphates (PPi) to deoxynucleosides or dNMPs, whereas the enzymatic method employs a phosphorylation process carried out by highly specific enzymes.
