Which method should I use for monoclonal antibody production, ascites or roller bottles?
Three major factors are used to determine the production mode: economics, ethical considerations, and the desire to define the environment in which the hybridoma cell line produces antibody. Economics: For most cell lines, the cost of antibody produced in ascites is less than the cost of antibody produced using roller bottles. This difference becomes very important when tens or hundreds of grams of monoclonal antibody are produced. Ethical considerations: Many individuals and many organizations choose to avoid the use of animals when a suitable alternative to the use of animals is available. All of SDIX’s animal facilities are AAALAC-accredited. Click here for more information about SDIX’s accreditations, registrations and licenses. Defining the environment in which the hybridoma cell line produce antibody: Ascites fluid is a complex mixture of many mouse proteins and other biomolecules. These molecules are present in the ascites product. The identities and concentrations of all of the
