Which measures are taken to ensure the reliability of DualChip GMO analyses?
All capture-probes are presented in triplicate on the DualChip GMO microarray. Built-in process control sets address every step including PCR, hybridization and detection. Controls are featured in the analysis summary and each is highlighted in yellow if it complies with the expected result. If a control is not correctly detected, a warning comment appears and the control name and warning statement are highlighted in red. Various controls and example warnings follow: • PCR control (PCRC): Consists of an external DNA spiked into the PCR reaction, coupled with its complementary capture-probes on the microarray. There are 3 different PCRC capture-probes, (1 per PCR) spotted twice in triplicate (resulting in 6 PCRC spots in total per PCR control). In the event that 1 PCRC of the 6 is not detected, the warning appears. • Positive hybridization control (PHC): Will hybridize with the Hybridization control spiked into the hybridization mixture. If the PHC is not detected, the warning appears.
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