Which M13/pUC primer should I use for my plasmid?
Click here to Enlarge Featured in this picture are the positions of the M1/-pUC sequencing primers on the pUC19 cloning site. The 3′ positions differ by 20 bases at most and will not significantly contribute to sequencing read lengths. If your plasmid is a M13/pUC derived plasmid, our in-house supplied M13/pUC primers should fit.