Which genes affect the penetrance of MLP?
What are some common motifs specific to adult phenotype mice which are not present in early phenotype mice? What are some common motifs specific to early phenotype mice not present in adult phenotype mice? Perform RFLPís on cDNA from a number of adult phenotype and early phenotype MLP-deficient mice in order to locate areas possibly related to the penetrance of MLP- deficiency. Use common motifs exclusive to each group as probes on a Southern blot of the cardiomyocyte DNA. Clone the motifs into plasmids and mutate the motifs one at a time with restriction-site manipulation mutation. Transform cells of the opposite phenotype with the mutated cDNA sequence and see if the phenotype changes. This could be done until a mutation in a gene caused a change in the penetrance. Are there proteins in striated muscle cells responding to mechanical stress? We know that hypertrophic response occurs after birth in early phenotype mice, probably due to increased mechanical stress after birth. Because M
