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Which filling volume should be selected for the cultivation of E. coli in a 250 mL shake flask without baffles?

April 26, 2017coli cultivation Filling flask ML Selected Shake volume

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Which filling volume should be selected for the cultivation of E. coli in a 250 mL shake flask without baffles?

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E. coli is a very respiratory active microorganism so the filling volume should not exceed 5 – 10 % of the total flask volume (i. e. 10 to maximum 25 mL in a 250 mL flask). Recommended culture conditions for the aerobic cultivation of E.