Which downstream applications can be used with tissue stored in RNAlater?
Tissue stored in RNAlater is compatible with all commonly used RNA isolation methods, including single reagent isolation products like Trizol®, and all of Ambion’s RNA isolation kits. It is also possible to extract both genomic DNA and total protein from samples stored in RNAlater. RNAlater will denature proteins, so it is only compatible with routine protein analyses such as Western blotting and 2D gel electrophoresis that do not require native protein [protocols] . Note: RNAlater is not recommended for use with tissues that will be sectioned using a cryostat.
