Which detection procedure (ECL or chromogenic) do you recommend for HRP Strep-Tactin® conjugates?
Both, chemiluminescene as well as chromogenic detection, are possible. Please note, that the application protocols for these procedures differ. The protocols are described in the “Western blot protocol” which is also available as pdf on the IBA download/tech info site. • What are the KD-values of the monoclonal antibodies? KD StrepMAB-Classic = approximately 1 M. KD StrepMAB-Immo: approximately 1 pM. These antibodies are monoclonal mouse antibodies, type IgG1. • Do the StrepMAB antibodies also work in immunoprecipitation and pull down assays? Yes, several applications are possible. We recommend to use the One-STrEP-tag (Strep-tag II is also possible) for the bait protein and to precipitate with Strep-Tactin Superflow or Strep-Tactin magnetic beads using biotin for the elution step. Alternatively, StrepMAB-Classic MacroPrep can be used for precipitation using the Strep-tag peptide for the elution step. Please note, if you have to use classical immunoprecipitation without an elution step
