Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

Which competent E. coli cells should I use to amplify the SureSilencing™ shRNA plasmids?

April 26, 2017amplify cells coli Competent plasmids shrna suresilencing™

0

Posted

Which competent E. coli cells should I use to amplify the SureSilencing™ shRNA plasmids?

1 Answer

0

Posted

Any molecular cloning-grade strain of competent E coli cells may be used to transform and amplify the SureSilencing™ shRNA Plasmids. They do not need to be a high efficiency cloning strain, because you only need to amplify a pure preparation of intact, supercoiled plasmid DNA.