Which combination of fluorochromes can I co-stain with eFluorTM Nanocrystal conjugated antibodies?
Combinations of fluorochromes which can be co-stained will be dependent on the filter setup of your instrument. With the proper filter setup, eFluorTM Nanocrystals are compatible with the following fluorochromes: FITC, Alexa Fluor 488, PE, PE-Cy5, PE-Cy5.5, PerCP-Cy5.5, PE-Cy7, APC, Alexa Fluor 647, Alexa Fluor 700, APC-Alexa Fluor 750, and Pacific Blue. The eFluorTM 605NC and eFluorTM 625NC conjugated antibodies cannot be used in combination with the PE-Texas Red fluorochrome as emissions are similar and excitiation does occur off the 488nm laser.
