Which combination of fluorochromes can I co-stain with eFluor 605NC, 625NC, and 650NC conjugated antibodies?
eFluor nanocrystal reagents can be used in combination with all other fluorochromes in our catalog. As with all fluorochromes, the instrument configuration will determine the fluorochrome use in the design of a multicolor staining panel. Please note that eFluor 605NC and eFluor 625NC reagents cannot be used in the same sample nor can they be used in a staining combination with a PE-Texas Red conjugate as the emission spectra are too similar to resolve. Also be aware that eFluor 650NC reagents will require compensation out of the APC detector.