Which columns can be used for trichothecene clean up?
Customer’s detailed question: In the case of trichothecenes, which column should be used for purification? What are the specific advantages of using the specialized columns for purified trichothecenes extracts? “Dark” alumina-charcoal columns, MycoSep®225 Trich or MyoSep®227 Trich+ columns, have better purification due to the added absorbent recovery for Trichothecenes. Typically, they recover 85 – 90% of the toxin. When using “white” silica columns, MycoSep®224 AflaZon or MycoSep®226 AflaZon+, you can obtain an 80-95% recovery (typically good trichothecenes recovery through the white columns will lose some clean up ability). The advantage of a clean up with MycoSep®224 AflaZon or MycoSep®226 AflaZon+ is that other toxins can be recovered from one extract in a single clean up step as well: One can analyse Zearalenone, Zearalenols as well as Aflatoxin B1, B2, G1 and G2 from the cleaned extract by e.g. using highly sophisticated LC-MS/MS methodology. The advantage of a clean up with Myco
