Which columns and magnets or separation program can be used with the Blood Dendritic Cell Isolation Kit II, human?
The Blood Dendritic Cell Isolation Kit II, human is a magnetic labeling system for the concurrent isolation of plasmacytoid and myeloid DCs from human PBMCs. The isolation is performed in a two-step separation with MACS® Technology. First, B cells and monocytes are magnetically labeled and depleted using a cocktail of CD19 and CD14 MicroBeads. Subsequently, the pre-enriched DCs in the non-magnetic flow-through are magnetically labeled and further enriched using a cocktail of antibodies against the DC markers CD304 (BDCA-4/Neuropilin-1), CD141 (BDCA-3), and CD1c (BDCA-1). It is recommended to use an LD Column for the depletion step and two MS Columns for positive selection. Using the autoMACSTM Separator or the autoMACSTM Pro Separator, it is recommended to use deplete05 and posseld2 for depletion and positive selection, respectively.
