Which channels will require compensation with eFluorTM Nanocrystal conjugated antibodies?
In general, the emission spectra of eFluorTM Nanocrystal conjugated antibodies are narrow, therefore resulting in minimal spillover into other channels and thus requiring minimal compensation. However, the eFluor 650NC conjugates do exhibit some spillover into the detectors for PerCP-Cy5.5 and APC and will require compensation for accurate data acquisition.
