Which buffers can I use in place of the Qdot® Incubation Buffer provided?
Qdot® nanoparticle conjugates have higher nonspecific binding in buffers that are not optimized for use with the materials. We have had successful staining results in a variety of buffer conditions, including TBS, PBS, RPMI media, and others, but have found that the performance in the Incubation Buffer is generally predictable and stable.