Which buffers and detergents are compatible with the eFluor® Nanocrystal conjugated antibodies?
Because you are working with directly conjugated antibodies, there should not be a need for detergents in your buffers. It is best to avoid their use if possible. We recommend using Tris Buffered Saline (TBS), pH 7.4 (Cat. No. 00-4954) rather than Phosphate buffered saline (PBS) for washing following binding of eFluor® Nanocrystal conjugated antibodies.