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Which are the modifications of Ziehl-Neelsens method?

method modifications
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Which are the modifications of Ziehl-Neelsens method?

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Decolourizer for Mycobacterium leprae is 5% H2SO4 Tissue sections containing Actinomyctes, Nocardia can be decolourized by 1% H2SO4 Cultures of Nocardia are decolourized by 0.5% H2SO4 Bacterial spores are decolourized by 0.25-0.5% H2SO4 Oocysts of Cryptosporidium are deceolourized by 10% H2SO4

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