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Where in my target sequence can I design qPCR primers?

design Primers qPCR sequence target
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Where in my target sequence can I design qPCR primers?

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The Applause™ WT-Amp System amplifies the entire transcript so primers can be designed at any location within the mRNA. In order to avoid interference from possible genomic DNA contamination, we recommend treating RNA with DNase and designing amplicons to span an intron.

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