WHERE ARE THE HISTONE PROTEINS LOCATED AND WHAT DO THEY DO?
H2B is one of four members of the Histone Tail Code family shown above that are involved in Nuclear Chromatin remodeling at the level of gene transcription. When appropriate a complex of DNA binding transcription factors bind to DNA and they will initiate transcription through opening of the DNA chromatin structure so that the DNA can initiate transcription of the genes being activated. Clearly, this molecular step in the pathology and pathogenesis of cancer is important and other scientists have verified it relevance to recurrence and progression of this devastating disease. SELDI-TOF IMMUNOASSAY: INCREASE OF H2B IN METASTATIC DUNNING TUMORS The Figure to the left illustrates the Detection of Histone H2B levels using SELDI immunoassay. Total cell extracts of G, AT-1 and MLL cell lines were tested for Histone H2B levels using the SELDI-based immunoassay with H2B antibody coated PS20 ProteinChip®arrays. (A)Gel view of the spectra obtained from anti-H2B coated PS20 ProteinChip®arrays on
