When working with DNA between 200-500 bp, should I use GENECLEAN or MERmaid?
A. When working with DNA 200-500 bp, you can use either the GENECLEAN or MERmaid Kit. For optimal results with the GENECLEAN Kit, add 1/10 volume of TBE Modifier or 1/200 volume of 10% acetic acid to the volume of NaI used in a particular prep. This will lower the pH of the NaI/GLASSMILK/DNA mixture to approximately 6.5, where smaller DNA species (down to 200 bp) will bind with maximum efficiency. No modifications are necessary for MERmaid.
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