When using Thermanox coverslips, which side of the coverslip is Cell Culture treated?
A simple method to determine which side of the coverslip is treated is the “droplet” test. A drop of water or culture medium will spread on the hydrophilic, treated side. The procedure is as follows: • Put a drop of sterile media or water on the coverslip. • If the droplet forms a bead, then the side of the coverslip is not treated. • If the droplet spreads evenly over the coverslip, the side is treated. The coverslips are packaged so the cell culture treated side faces the label.