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When using different miScript Primer Assays with the miScript PCR System, is it necessary to optimize reaction conditions for each miScript Primer Assay?

April 26, 2017assays Conditions different miscript necessary optimize PCR primer REACTION system

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When using different miScript Primer Assays with the miScript PCR System, is it necessary to optimize reaction conditions for each miScript Primer Assay?

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It is usually not necessary to optimize reaction conditions for each miScript Primer Assay. However, if the miRNA has a high GC content, increasing the annealing temperature (e.g., increase from 55°C to 57°C) could improve results.