When using CloneSaver for plasmid work, why doesn the genomic DNA of the host bacterial cell contaminate the plasmid sample?
Some genomic DNA may be present with the plasmid during analysis, but the gDNA should not contaminate the analysis or affect the results. Both the genomic DNA and the plasmids will bind to the FTA upon sample application. During the washing steps, the cell debris will get washed of, leaving the gDNA and plasmids entrapped within the fibres. If the punch is then used for analysis, the gDNA will be present. If, however, the plasmid is eluted off the punch first, the bacterial genomic DNA will not elute (only eluting in TE for 5 minutes at room temperature) and not be present for analysis.
