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When using both L-Photo-Leucine and L-Photo-Methionine, do I need to add the native analogs as well?

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When using both L-Photo-Leucine and L-Photo-Methionine, do I need to add the native analogs as well?

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No, only supplement DMEM-LM medium with the photo-reactive amino acids. Adding native analogs will compete for incorporation, thereby reducing crosslinking efficiency.

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