Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

When transducing, is it best to reduce the volume of media on my cells?

April 26, 2017best cells media reduce transducing volume

0

Posted

When transducing, is it best to reduce the volume of media on my cells?

1 Answer

0

Posted

Many researchers prefer to reduce the volume of media on their cells when performing a transduction given their cell line is not adversely affected. A reduction in volume can boost the overall concentration of the lentivirus on the cells, thus potentially increasing transduction efficiency.