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When staining cells or spheroids in the sponge, how problematic is background staining due to the AlgiMatrix™?

April 26, 2017algimatrix™ background cells problematic spheroids sponge staining

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When staining cells or spheroids in the sponge, how problematic is background staining due to the AlgiMatrix™?

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Answer. There is virtually no background staining with alginate. The same surface characteristic that prevents cells from adhering also makes it very difficult for molecules such as fluoresceinated antibodies to adhere, thus essentially no background staining.