When should I use an expression clone with / without stopcodon?
Many ORFs are available with or without stopcodon – see the respective annotation in your GenomeCube search hit. The availability of both formats provides the freedom of choice to produce native proteins (closed sequence / with stopcodon) or C-terminal fusion constructs (open sequence / without stopcodon). Please take care not to combine a clone with stopcodon and a vector with C-terminal tags, since the tags will not be expressed. In case you choose a clone without stopcodon and a vector without C-terminal tags the translation will be randomly terminated by a stopcodon located in frame in the vector sequence, so that the expressed protein will comprise additional amino acids at the C-terminus. To derive the sequence of a particular imaGenes ORF Expression Clone you may contact our Product Support Department.
