When should I use a Taq DNA polymerase?
If you want to amplify DNA fragments efficiently, you should use a Taq DNA polymerase. Z-PCR Mix, Direct-Load PCR Mix and Z-PCR Mix GC-Rich contain Taq DNA polymerase at the optimized concentration which can give a high efficiency and yield. Nonproofreading enzymes such as Taq DNA polymerase can give you minimum errors and maximum efficiency under appropriate high-fidelity conditions. High-fidelity conditions include low magnesium and dNTP concentrations (<50µM), and short extension times. It is very hard to beat Taq DNA polymerase for the combination of specificity, sensitivity, and yield. Return to Top When should I use proofreading enzymes such as Pfu, Pwo, Vent or Deep Vent? If you need a short fragment from a plasmid, use a high template ( i.e ., ng of plasmid) and keep the cycle number low (10 or 15), then it is fine to use proofreading DNA polymerases. However, you can not assume that the PCR product will have 100% fidelity just by using a proofreading DNA polymerase. No DNA pol
