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When should 2D DIGE gels be stained with Deep Purple?

Deep dige gels Purple stained
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When should 2D DIGE gels be stained with Deep Purple?

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A. 2-D DIGE gels should not be fixed or stained with Deep Purple until after all CyDye scans have been completed. Fixing before scanning for CyDyes will effect quantitation and cause a high background.

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