When pipetting with the Biomaster, air is drawn into the tip. Is the pipette leaking?
It’s unlikely that the pipette seal is faulty. More likely is that the tip seal is faulty. If the pipette is pressed down too hard when attaching tips from the rack, the leakage seal of the piston in the tip can be negatively affected. This is considerably more noticeable with highly viscous solutions than with fluid solutions. In addition, the tips may not be autoclaved.
