When it comes to beta counting, what is the difference between the use of a discrete sample liquid scintillation counter and a flow-through monitor for counting HPLC eluates?
In a liquid scintillation counter, fractions of the eluate are pipetted into glass vials, mixed with liquid scintillator solution and automatically moved into a counting chamber between two photomultipliers operating in coincidence, where a static measurement of several minutes duration is made. In a flow- through monitor, eluate leaving the HPLC system is either (a) continuously mixed with liquid scintillator and the mixture passed through a Teflon coil cell or (b) passed through a Teflon coil cell packed with insoluble solid scintillator; in either case the coil is positioned between two photomultipliers. The solution leaving the cell is most often directed to a waste container but is sometimes sent to a fraction collector for later use.
Related Questions
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