When imaging a SYPRO™ Ruby stained gel, can I still image for the original CyDye™ DIGE Fluor minimal dyes and get three (or four) related images?
No. SYPRO Ruby has very broad excitation and emission wavelengths. Once the gel has been stained with SYPRO Ruby, if you try and scan again using CyDye DIGE Fluor parameters, the SYPRO Ruby will be excited by the 532 nm laser (used for Cy™3) and emit light, interfering with quantitation in the Cy3 and Cy5 channels.
Related Questions
- When imaging the SYPRO™ Ruby stained gel, can I still image for the original CyDye™ DIGE Fluor minimal dyes and get three (or four) related images?
- When imaging a SYPRO™ Ruby stained gel, can I still image for the original CyDye™ DIGE Fluor minimal dyes and get three (or four) related images?
- Why can’t I pick directly from a gel containing CyDye™ DIGE Fluor labelled proteins?
