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When I use a new TSKgel SW/SWxl column I get low recoveries of my protein. How can I avoid this?

April 26, 2017avoid column low protein recoveries SW swxl tskgel

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When I use a new TSKgel SW/SWxl column I get low recoveries of my protein. How can I avoid this?

1 Answer

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There are active sites on the resin that require conditioning with either your sample protein or another material such as bovine serum albumin (BSA). For the TSKgel SW/SWxl column we recommend 4 successive injections of 100µg BSA to condition the resin. The TSKgel PW/PWxl sizing columns are far less hydrophilic and normally do not require conditioning.