When do I need to use DNARelease™ Additive?
DNARelease Additive is especially important in Direct PCR from mouse samples. In the Direct PCR protocol it is added to the loading buffer for gel electrophoresis. This will prevent the PCR products from getting trapped in the agarose gel wells. In the dilution protocol for mouse samples, DNARelease is added to the TE buffer in order to aid releasing the DNA from the sample. See Phire® Animal Tissue Direct PCR Kit. If you notice that the DNA sample is stuck in the wells with other sample material, adding DNARelease to the loading buffer may be helpful.
