When do I need N-terminal acetylation or C-terminal amidation?
The reason for adding these modifications is to avoid unnatural charges at the ends of the peptide, and to make the peptide more resistant to exopeptidases. We suggest to amidate the C-terminus of N-terminal peptides. The COOH-group then remains uncharged like it would be as part of a polypeptide chain. Analogously, the N-terminus of C-terminal peptides should be acetylated. “Internal” peptides should be modified at both ends.
