When can baby chicks climb ladders?
Don’t take the risk. The nest should have been kept at ground level the entire brooding period. You should still separate- if you provide an enclosure within the current enclosure, the lone hen will still be able to interact with her sister and prevent loneliness. The last time I ran out of broody boxes and decided to try to raise chicks in the coop, one of the adult hens went on a rampage a few weeks after the hatching and I had to put down two chicks with badly broken legs. It wasn’t a nice thing to have to do. Never take risks, even those you think are “kind”, put the chicks first.