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When adapting cells from serum-containing to serum-free media, is the direct or sequential adaptation method better?

April 26, 2017adaptation adapting cells media method Sequential serum-containing serum-free

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When adapting cells from serum-containing to serum-free media, is the direct or sequential adaptation method better?

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The sequential adaptation method is recommended. When cells are transferred to serum-free medium from a serum-rich environment, the cells are deprived of components in the serum-rich environment. Sequential adaptation allows the cells to gradually adapt to their new serum-free environment.