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Whats the difference between PE/Cy5 and PE/Cy5.5 conjugates?

April 26, 2017conjugates cy5 PE

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Whats the difference between PE/Cy5 and PE/Cy5.5 conjugates?

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Both PE/Cy5 and PE/Cy5.5 are excited at 488 nm with an argon laser, but the emission of PE/Cy5 peaks at 670 nm and the emission of PE/Cy5.5 peaks at 690 nm. When used together with APC, PE/Cy5.5 requires less cross beam compensation than PE/Cy5.