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Whats the best way to adjust the pH of a mobile phase?

April 26, 2017best mobile pH phase

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Whats the best way to adjust the pH of a mobile phase?

1 Answer

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10 Posted

Arguably, the best way is not to attempt to adjust pH at all, but rather to prepare the mobile phase by weight. Weighing is much more precise (a 20% error in weighing translates to only a 0.1 unit error in pH). You can find an on-line calculator for buffers here (http://www.bi.umist.ac.uk/users/mjfrbn/buffers/makebuf.