Whats the advantage of using adenoviral transfection?
The virus-mediated RNAi may circumvent some of the problems associated with cells that are generally refractory to RNAi, such as nontransformed primary cells, e.g. fibroblasts and dendritic cells. • Why do you set the default length to 21-mer instead of 19-mer? A: 21-mer is better based on the following reference: Harborth J, Elbashir SM, Vandenburgh K, Manninga H, Scaringe SA, Weber K, Tuschl T. (2003) Sequence, chemical, and structural variation of small interfering RNAs and short hairpin RNAs and the effect on mammalian gene silencing. Antisense Nucleic Acid Drug Dev. 2003 Apr;13(2):83-105. • Which inducer is better? Tetracycline or doxycycline? A: Doxycycline is better, because it is more stable in the medium. Tetracycline can be degraded easily. • Why do you add one more C after BamHI site in the siRNA insert? A: The transcription of pol III promoter starts at position 8. That extra C is to make sure that the siRNA insert gets transcribed correctly. • Why is my lentiviral virus ti
