Whats Next after pGLO™ Bacterial Transformation?
(2 hours) Don’t stop at cloning the gene — identify the protein responsible for the green fluorescence! Take white and green colonies from your transformed plates, prepare sample lysates and identify the pGLO protein using polyacrylamide gel electrophoresis. The bacterial proteome contains thousands of proteins, but only the cloned GFP protein glows! Use the phenotypic trait of this protein to quickly identify the protein within the complex mix. DNA > RNA > PROTEIN > TRAIT — Green Fluorescence! Science Education for New Civic Engagements and Responsibilities (SENCER) Tuesday, 18 August at San Francisco State University Session length: half-day Organizer: Amy Schacter (Associate Provost, Office of Research Initiatives, Santa Clara University, Santa Clara, CA; 408-551-7041, ashacter@scu.edu). Co-organizer: Steve Bachofer (Department of Chemistry, Saint Mary’s College); 925-631-4694, bachofer@stmarys-ca.edu). Description: Initiated in 2001, Science Education for New Civic Engagements and
