What yield of immunoprecipitated DNA can I expect with the kit?
The results of our studies show that for the histones we can expect between 10-20 ng of DNA from 1mln cells. However the yield will vary depending on starting number of cells, antibody quality, the target – transcription factor or histone modification, and the quality of the starting sample material. Please also note that the amount of DNA that is immunoprecipitated does not necessarily indicate the percentage of specific to non-specific DNA.
