What would happen if I used home-made PCR master mixes or master mixes from other manufacturers with the RT² qPCR Products?
We can only guarantee the performance of RT² qPCR Primer Assays with our RT² qPCR Master mixes. Our master mix components and primer design algorithm were optimized together to guarantee production of single bands of the predicted size. When we do test other sources of master mix with our Primer Assays, we frequently see primer dimers and other non-specific products that confound SYBR-Green based qPCR detection.
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