What version of GUSPlus is used is used in pCAMBIA vectors?
pCAMBIA1105.1, 1105.1R, 1305.1 and 1305.2 use a fully synthetic, codon and expression-optimized version of a glucuronidase-encoding gene from a Staphylococcus species isolated by CAMBIA. These plasmids are available under CAMBIA’s BiOS license. In pCAMBIA1305.2 the GusPlus gene is fused to a sequence encoding the glycine rich protein secretion signal peptide. The secretion signal results in the GUSPlus protein being efficiently transported to the periplasmic (or apoplastic) space, where it is active. This allows non-destructive in vivo staining and detection in living tissues that can be grown further after staining. A castor bean catalase gene intron inserted near the 5′ end of the GUSPlus gene eliminates expression of GUSPlus in bacteria, but allows expression upon transfer to a plant cell. This make monitoring of gene expression associated with transformed tissues more informative and less liable to artifactual intepretation. The presence of the intron also improves the overall expr
