What types of expression data are found in EMAGE?
A. EMAGE contains in situ gene/protein expression data, assayed using in situ hybridisation (ISH), immunohistochemistry (ISR) and in situ reporter (ISR) assays. The emphasis is on endogenous expression data: for ISH and IHC data, specimens are usually wild-type or occasionally heterozygous mutants (but with an apparently wild-type phenotype). For ISR (knock-in reporters or gene trap data), specimens are usually heterozygous mutants (but with an apparently wild-type phenotype) and occasionally homozygous mutants (again, with an apparently wild-type phenotype). Very occasionally we incorporate data from transient transgenic specimens under the proviso that the staining pattern reflects the endogenous gene expression pattern.
