What Types of Chromatography Are Used?
Flash chromatography is a type of preparative liquid chromatography used for the separation of organic compounds. This is adsorption chromatography for the routine purification of organic compounds. By using the flash technique chromatographers can scale up normal phase chemistries from thin layer chromatography (TLC) helping to satisfy the demands of the pharmaceutical and biotech industries in the transition to large scale purification of organic compounds and peptides. The technique utilizes an air pressure driven hybrid of medium pressure and short column chromatography optimized for particularly rapid separations.1 Flash is very similar to traditional column chromatography except that solvent is driven through the column by applying positive pressure. Resolution is measured in terms of the ratio of retention time (r) to peak width (w, w/2). The technique simply uses a set of chromatography columns and flow controller valves. Modern flash chromatography systems are very convenient,
