What type of proteins express well in K. lactis?
Many types of proteins can be secreted from yeast cells. As a general rule, those that tend to secrete best are proteins that are also secreted by their native host (e.g. glycosidases, serum albumins, cytokines, etc). However, there are numerous examples in the literature of normally non-secreted proteins that have been successfully secreted from various yeasts. Therefore, when in doubt, it is always best to try secreted expression. Additionally, intracellular protein expression in yeast is also possible for a wide range of proteins and is a great alternative to bacterial protein expression.
